Clostridioides difficile is the most important pathogen causing antimicrobial-associated diarrhea and has recently been recognized as a cause of community-associated C. difficile infection (CA-CDI). This study aimed to characterize virulence factors, antimicrobial resistance (AMR), ribotype (RT) distribution and genetic relationship of C. difficile isolates from diverse fecally contaminated environmental sources. C. difficile isolates were recovered from different environmental samples in Northern Germany. Antimicrobial susceptibility testing was determined by E-test or disk diffusion method. Toxin genes (tcdA and tcdB), genes coding for binary toxins (cdtAB) and ribotyping were determined by PCR. Furthermore, 166 isolates were subjected to whole genome sequencing (WGS) for core genome multi-locus sequence typing (cgMLST) and extraction of AMR and virulence-encoding genes. Eighty-nine percent (148/166) of isolates were toxigenic, and 51% (76/148) were positive for cdtAB. Eighteen isolates (11%) were non-toxigenic. Thirty distinct RTs were identified. The most common RTs were RT127, RT126, RT001, RT078, and RT014. MLST identified 32 different sequence types (ST). The dominant STs were ST11, followed by ST2, ST3, and ST109. All isolates were susceptible to vancomycin and metronidazole and displayed a variable rate of resistance to moxifloxacin (14%), clarithromycin (26%) and rifampicin (2%). AMR genes, such as gyrA/B, bla_CDD-1/2, aph(3′)-llla-sat-4-ant(6)-la cassette, ermB, tet(M), tet(40), and tetA/B(P), conferring resistance toward fluoroquinolone, beta-lactam, aminoglycoside, macrolide and tetracycline antimicrobials, were found in 166, 137, 29, 32, 21, 72, 17, and 9 isolates, respectively. Eleven “hypervirulent” RT078 strains were detected, and several isolates belonged to RTs (i.e., RT127, RT126, RT023, RT017, RT001, RT014, RT020, and RT106) associated with CA-CDI, indicating possible transmission between humans and environmental sources pointing out to a zoonotic potential.

The goal of this study was to investigate the vegetation composition and structure of the Al-Orban area in Gharyan district, Libya over two continues growing seasons from the first of January 2020 to the first of Jaunary 2022. During this period, a total of 309 plant species were identified and collected in the field. These species belonged to 43 families, with 39 families of dicotyledons and 4 families of monocotyledons. The most dominant family was Asteraceae with 60 species, followed by Fabaceae with 32 species, Poaceae with 33 species, and Brassicaceae with 35 species. The most dominant genera were Plantago and Euphorbia, each represented by 6 species, followed by Erodium and Chenopodiun with 5 species each, and Astragalus, Centaurea, Rumex, Concolvulus, and Stipa each represented by 4 species. Life-form spectrum analysis indicated that Therophytes were the most predominant with 189 species, followed by Hemicryptophytes with 47 species, and geophytes with 40 species. Furthermore, chorotype spectrum analysis showed the dominance of Mediterranean species with 128 species, followed by Mediterranean/Iranian-Turanian with 66 species. These findings provide valuable insight into the plant diversity and distribution in the Al-Orban area of Gharyan district, Libya.

Abstract

The research was conducted to discuss the impact of storytelling and theater in building a remarkable personality and moral values in children. Furthermore, it has been mentioned the importance of preschool period and educated family to help of building the child’s personality and cultivating moral values. In addition, this research has been mentioned that in Libya there is not separation between moral education, strong personality and religion, which has been gotten all his values from second language and it has been explained and clarified the character development.

Moreover, it has been discussed two main headlines, ‘the storytelling and the child’s early age’ and ‘the role of storytelling and theater to create a creative and strong personality.

 Besides, some tricks and plays techniques have been mentioned to enhance the creativity and remarkable personality among children, (pretend play / language play /musical play and play drawing and writing).

Finally, some advices and recommendations have been given to the parents and teachers in order to guide them to the appropriate ways to establish the child’s personality and moral values. 

Abstract: This review aimed to investigate the effect of pH on morphology, and size of gold nanoparticles (AuNPs) biosynthesized using extracts of various plant parts and agricultural waste. By reviewing previous studies, it was found that the formation of AuNPs was more rapid in neutral and basic mediums than in acidic mediums. Furthermore, most of those studies indicated that the smallest sizes of biosynthesized AuNPs, produced by plant extracts, were in neutral and alkaline mediums. Regarding the morphology, it was found that multiple shapes of the AuNPs were obtained in an acidic medium of the reaction, while there was typically a dominant shape of the AuNPs in the basic medium. In conclusion, significant quantities of AuNPs with appropriate sizes and morphology could be obtained by controlling the medium of the reaction when AuNPs are biosynthesized using extracts of plant parts and agricultural waste.

Derivative-free optimization techniques are widely used for solving optimization problems. The

focus in this work is given to some variants of Nelder-Mead and particle swarm methods to tackle two

unconstrained optimization problems originated from optimal control, namely the pole assignment problem for

discrete and continuous-time positive systems. we present the Nelder-Mead and Particle Swarm optimization

methods to solve problems. Moreover comparing our results with benchmarks in the literature.

The extremely high speed offered by UWB systems allows the guard band to be extended to 100% instead of maximum of 25% as in broadband systems. This particular guard ensures this channel is completely free of spurious signals before transmitting next symbol. In this paper, the performance of non block transmission frequency domain UWB receiver with changeable zero-padding is presented. The receiver can be an alternate to Rake receivers, since clustering effect, common in UWB signals makes Rake receivers impractical and imposes low complexity rake receivers that are not optimum, either.

The problem of microbial resistance continues to increase in all regions of the world, and even though a very large number of antibiotics have been produced in the last 30 years, microbial resistance is maintained because of the wide use of these drugs against many infectious diseases. Thus, natural products are seen as the alternative solution. Based on previous studies, Peganum harmala has been reported to exhibit several biological properties including antibacterial and antifungal. Thus, the main aim of this study is evaluating antibacterial activities of P. harmala against Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus, which isolated from infected patients in (Alassaba'a hospital, Alassaba'a, west of Tripoli). Agar disk diffusion technique was used for detecting the antibacterial activity, and the minimum inhibitory concentration was tested by serial dilution methods. The results showed that the growth of all tested bacteria were inhibited strongly by methanol extract of seeds at 1.0% with inhibition zones 14.66, 12.00 and 15.33mm against E. coli, k. pneumoniae and S. aureus respectively. Besides, MIC values ranged between 0.625 to 1.25 mg/mL. While, MBC values ranging between 1.25 to 5 mg/mL. On the other hand, methanol extract of leaves was also inhibit the growth of E. coli, k. pneumoniae and S. aureus with inhibition zones 11.00, 9.66 and 10.00mm respectively. As well as, MIC values ranging between 1.25 to 2.5 mg/mL, while MBCs value ranging between 2.5 to 5 mg/mL . In conclusion, methanol extract of P. harmala seeds exhibited strongly antimicrobial activity against E. coli, k. pneumoniae and S. aureus, thus it can be developed as anti-bacterial agent.

Abstract

Background: Cutaneous leishmaniasis (CL) is endemic in many regions of the world, and has a significant economic and health impacts. Despite the numerous proposed therapies for this zoonotic parasitic disease. Currently, there is no definitive cure for CL. Meglumine antimony compounds are regarded as the typical treatment for leishmaniasis. However, they have a rather high incidence of adversative effects, that always, are not successful. Alternatively, Paromomycin and dapsone were used in various studies, though, with conflict results. Aim of study: This study aims to evaluate the clinical efficacy and safety of using of paromomycin cream, oral dapson and their combination in treatment of CL. Patients and Methods: This study is a prospective randomized clinical trial was carried out at the private outpatient Al- Ahlam Center of Dermatology and Cosmetology in city of Gharyan, Libya. That started from 1st of January 2020 to end of Dec 2021. 42 patients were joining this study, and randomly were divided into three groups according to protocol of treatment to assess the clinical response of the treatment methods. Results Regarding the outcome of the three different treatment regimen and after exclusion of the patients stop their follow-up; we found that 50% of dapsone treated group cleared the lesions in 1-2 months versus only 40% in paromomycin & Dapsone treated group and 0% for the paromomycin group, only 8 patients had no improvement with received treatment; 4 (33.3%) from dapsone group, 2 (20%) from each other group (P=0.036) Conclusion: Using of paromomycin & dapson combination in treatment of cutaneous leishmaniasis shows better result and early clearance LC lesions, whereas, using of topical paromomycin cream or oral dapsone alone were effective. Also, our results showed that, paromomycin is effective, safe and well tolerated in children. Keywords: Cutaneous leishmaniasis, Paromomycin, Dapson, Gharyan, Libya

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