Background/Objectives: Clostridioides difficile is a “One Health” pathogen and a cause of antibiotics-associated diarrhea and pseudomembranous colitis. Mobile genetic elements (MGEs) have been documented in the genomes of clinical C. difficile strains; however, the presence of MGEs in environmental strains remains poorly characterized. Thus, the present study was conducted with the objective of identifying the prevalence of MGEs, including mobilizable transposons (MTns), conjugative transposons (CTns), plasmids, and insertion sequences, in whole genome sequences (WGSs) of environmental C. difficile isolates. Methods: The analysis of MGEs was conducted using 166 WGSs obtained from C. difficile strains isolated from various environmental sources contaminated with feces. The MGEs were identified using bioinformatic tools. Results: A total of 48.2% (80/166) of the studied genomes were identified to harbor nine transposons, including Tn916, Tn6194-like, Tn5397, Tn6215, Tn4001, Tn6073, Tn6110, Tn6107, or Tn5801-like. The majority of MTns and CTns could be found within C. difficile sequence types ST11, ST3, and ST35. The results demonstrated close genetic relatedness among the studied genomes, the array of antimicrobial resistance (AMR) genes, such as tetM, ermB, and aac(6′)-aph(2″), and the presence of CTns. Furthermore, the analysis revealed that 24.7% (41/166) of the genome sequences of isolates were associated with various predominant plasmid groups, including pCD6, pCD-ECE4-6, pCD-WTSI1-4, pCDBI1, and pCd1_3, which belonged to 16 different sequence types. Furthermore, several plasmids were identified as harboring the prophage phiCDHM19. Conclusions: The results of the current study suggest that the identified plasmids are abundant and may encode functions that are relevant to C. difficile physiology. The genomes of C. difficile strains examined contain closely related CTns, suggesting that horizontal transfer of AMR is important in this species or other bacterial species. Further research is required to ascertain the effect of these genetic elements and their transferability on the biology of C. difficile.

Abstract. The cosmopolitan or banded garden spider, Argiope trifasciata (Forskål, 1775), the lobed spider, A. lobata (Pallas, 1772) and the MENA lobed spider, A. sector (Forsskål, 1776) are reported, figured and described for the first time in Gharyan city, northwestern of Libya, based on adult female specimens gathered from different plants:- amaranth, haloxylon, prickly saltwort, retama, safflower, vetches, across various sites in the city. Unpublished reports of Argiope species were recorded in 1934 along the Libyan coast.

ABSTRACT

Argemone ochroleuca Sweet, Brit. Fl. Gard. Ser.1 3(2): pl. 242 (1828) of the family Papaveraceae is reported here for the first time from Libya. This species is native to Mexico. Flowering specimens of A. ochroleuca were collected from the region Ariggiba (26°35’11.8”N 13°29’24.8”E), 110 km southwest of the city of Sabha, which lies about 700 km south of Tripoli. It is presented with updated nomenclature, taxonomic description, geographical distribution, key to genera, location in Libya, and colour photographs taken in the field. The voucher specimens are deposited in the Herbarium of Botany Department (ULT), University of Tripoli, Libya.

مؤخرًا برزت عدة تساؤلات حول طبيعة علاقة الذكاء الاصطناعي بالذكاء البشري، فالهدف من هذه الدراسة هو مناقشة فرضية أن العلاقة بين الذكاءين يجب أن تُبنى على التكامل لا على التنافس. واعتمدت الدراسة على المنهج الوصفي التحليلي من خلال مراجعة الأدبيات الحديثة التي تناولت الإمكانات التكاملية بينهما.. كما واوصت الدراسة بتبني اطار تعاوني تشاركي (الذكاء التشاركي) والذي يعزز التفاعل الايجابي بين الانسان والآلة..

Abstract

Dental caries is a widespread infectious disease influenced by bacterial activity, dietary factors, and host

susceptibility. Community water fluoridation has been a key public health measure for caries prevention,

particularly in children. The optimal fluoride concentration in drinking water (0.7 mg/L) balances caries

reduction with minimal fluorosis risk. This study assessed fluoride levels in drinking water and their

correlation with dental caries in primary school children in Gharyan, Libya. A cross-sectional study was

conducted on 350 children (aged 7–10 years) from urban and rural schools in Gharyan. Water samples from

five stores and one groundwater source were analyzed for fluoride concentration using an ion-selective

electrode. Dental caries was assessed using the dmft/DMFT index. Fluoride levels in water stores were

negligible (0.0–0.1 mg/L), while unfiltered groundwater exhibited high concentrations (6.0–7.0 mg/L),

exceeding WHO guidelines (1.5 mg/L). The low fluoride availability in most drinking sources suggests

limited caries-preventive effects from water fluoridation in this region. The study highlights insufficient

fluoride exposure in Gharyan’s public water supply, potentially contributing to higher caries prevalence.

Further research is needed to evaluate alternative fluoride delivery methods and assess groundwater safety

to optimize oral health outcomes.

Keywords. Fluoride Concentration, Drinking Water, Dental Caries, Primary School

SUMMARY This research documents the initial finding of Chloris pycnothrix Trin., frequently referred to as spiderweb chloris, in Libya, particularly from the Ariggiba region close to Sabha city. This grass, originating from tropical and subtropical regions, is versatile and may become invasive. It was recognized via morphological examination during field surveys in 2023–2024, with samples stored in a herbarium. The species flourishes in alluvial plains as well as in disturbed habitats like road shoulders. It can be mistaken for Chloris virgata, although they differ in some important morphological aspects. It was previously believed that only one species of Chloris existed in Libya. This study provides a revision and an identification key for them in the context of Libyan flora. The tolerant nature of C. pycnothrix to drought, combined with its ability to colonize poorly maintained land, makes its potential introduction—most likely through anthropogenic means—alarming ecologically and emphasizes the need for management strategies to contain its spread and monitor its impact.