Abstract

Natural dentin repair is a biologically mediated process that preserves tooth vitality through odontoblast activity and intracellular signaling pathways. This study employed a mixed methods design, combining laboratory experiments with descriptive analytical surveys of dentists in Egypt, to investigate the mechanisms of odontoblast differentiation and assess clinical knowledge and practices related to regenerative dentistry. Laboratory analyses included microscopic and molecular techniques to evaluate cellular signaling during dentin repair, while questionnaires were distributed to 100 dentists across public and private sectors. Data were analyzed using quantitative statistics, qualitative assessments, and integrative interpretation of laboratory and clinical findings. Results revealed significant positive correlations between dentists’ knowledge of intracellular signaling and their clinical practices, with Spearman correlation coefficients ranging from 0.52 to 0.65 (p = 0.01). A majority of respondents (80%) reported experience with mesenchymal stem cell therapy for craniofacial bone remodeling or dental tissue regeneration, and 70% observed improvements in bone density, while 65% reported enhanced dental tissue regeneration. Imaging studies were widely used, with 85% of participants undergoing radiographic or CT evaluations to monitor progress. Despite these encouraging outcomes, only 35% of dentists were familiar with molecular techniques targeting signaling pathways, and just 40% reported applying clinical strategies to enhance odontoblast differentiation. Notably, 75% expressed interest in specialized training to improve their knowledge and practices in regenerative dentistry. The study concludes that while theoretical awareness of dentin repair mechanisms is relatively strong, clinical application remains limited. Bridging this gap through structured training and evidence-based protocols will be essential for integrating molecular insights into routine dental practice, thereby improving dentin repair outcomes and long-term tooth vitality.

This paper presents the design and implementation of a smart robotic vacuum cleaner based on Internet of Things (IoT) technologies, named IO-VAC, specifically developed for smart home environments. The paper covers both the mechanical and electronic aspects of the robot’s development. A 2D layout was created using ArchiCAD to define the positioning of components and sensor angles when designing the robot, followed by a 3D model to visualize the final design. The robot chassis was fabricated using reinforced plastic to ensure durability and lightweight structure. The IO-VAC is controlled using an Arduino Mega board programmed in C/C++. Where, the system includes three ultrasonic sensors for obstacle avoidance, along with an infrared (IR) sensor for edge detection to prevent falls. The embedded system of IO-VAC integrates an ESP8266 Wi-Fi module, allowing for remote control via the Blynk IoT platform to enable users to control the robot’s movement (forward, backward, left, right), adjust suction power, select the desired cleaning algorithm, and monitor the battery level in real-time. In this paper three movement algorithms were developed: random, spiral and zigzag, and their performance was tested on two types of surfaces smooth and rough to ensure reliability and efficiency in various cleaning conditions after testing the validation of IO-VAC. Based on the test results of robot performance, the zigzag algorithm proved to be the most effective in terms of covered area, cleaning mission time, power consumption and minimizing redundancy. This work demonstrates an effective integration of embedded systems with IoT technologies, offering a smart and cost-efficient solution for automating household cleaning tasks.

Abstract

The proper management of biological waste in laboratory settings is a critical aspect of biosafety in microbiological and biomedical laboratories. Beyond protecting lab workers, biological waste management safeguards public health, protects the environment, and ensures compliance with legal and regulatory frameworks. Segregation, decontamination of liquid waste, decontamination of solid waste, and techniques for inactivating toxins are all main aspects of biological waste management that will be outlined, aligned, and summarized with the main institutional biosafety protocols for biological waste management, published by major authorities, including CDC and WHO. This research found that the information from the two institutions complements each other. Therefore, this brief review aimed to compile and unify information from both institutions to construct a single, coherent text that provides a practical summary for medical laboratory professionals seeking to enhance the implementation of international, harmonized protocols that strengthen biosafety, environmental stewardship, and regulatory compliance

Despite the critical demand for advanced English proficiency in Libyan 

higher education, primary public schools, particularly in rural areas, have 

been neglected. This qualitative study investigates the imperative for 

implementing improved methodologies in Teaching English as a Foreign 

Language (EFL) in Libyan primary public schools, using the post-conflict 

rural municipality of Al Gwaleesh as a case study. Data were collected by 

questionnaires and classroom observations from ten teachers and ten third

year pupils. The findings reveal a systemic reliance on traditional Grammar

Translation Methods, a stark deficiency of resources (including basic 

teaching aids and ICT), and minimal student engagement. Teachers 

demonstrated limited application of Communicative Language Teaching 

(CLT) principles, focusing predominantly on rote memorization and written 

exercises. Consequently, pupils reported low motivation and a failure to 

grasp the language's practical utility. The study concludes that a 

fundamental overhaul of EFL pedagogy in Libya is urgently required, 

recommending the integration of audio-visual aids, activity-based learning, 

and 

strategic 

incorporation 

of 

Information and Communication 

Technologies (ICTs) to foster intrinsic motivation and build a sustainable 

foundation for English proficiency.

Antimicrobial Susceptibility Testing (AST) is essential for guiding appropriate antibiotic therapy. The disk diffusion method is widely used in Libya due to its low cost, yet deviations from standardized protocols can compromise result accuracy, affecting clinical decisions and patient outcomes. This study evaluated the performance of the disk diffusion AST method in medical laboratories in Gharyan, Libya, using EUCAST guidelines (version 12.0) as a benchmark. Seventy-five AST plates representing 122 clinical specimens were randomly collected from three pathology centers between September and November 2025. Plates were analyzed for the type of culture media, number of specimens per plate, number of antibiotic disks, and readability of results. All plates were 90 mm in diameter. Nutrient agar was used for 118 specimens, and blood agar for two; none used Mueller-Hinton agar as recommended. Only 17% of plates tested a single specimen per plate; the majority tested one specimen on half or a quarter of a plate. The number of antibiotic disks per specimen often exceeded EUCAST limits (up to 20 disks per plate), and only 8% of plates yielded readable results. Widespread deviations from EUCAST protocols—including inappropriate media, specimen overloading, and excessive disks—compromise AST reliability. These errors may lead to incorrect antibiotic prescriptions, delayed recovery, and increased antimicrobial resistance. Strict adherence to standardized guidelines is urgently required to ensure accurate, clinically meaningful AST results. Moreover, raising awareness of the importance of following standard guidelines is crucial for correcting the course