Abstract

The study aimed to determine the prevalence of vitamin D and calcium deficiency in a sample of residents in the city of Gharyan, located in the western regions of Libya, based on 300 cases visiting laboratories and clinics and medical clinics in the city for the year 2025 AD, divided into two groups for both sexes with a number of (150) cases, for different age groups from (5-85) years The study reached results showing that vitamin D and calcium deficiency is widespread among cases for both sexes, as the percentage of vitamin D deficiency in males reached 29.34%, and in females it reached 25.66%, calcium deficiency was recorded at 25.34% in males and 24.66% in females. The highest prevalence of vitamin deficiency was recorded in the age group of (46-65) years, at 20.66% in males, and 25.33% in females. Followed by the age group from (26-45) years, with 29% of the total cases, 14.33% in males, and 14.66% in females. This is consistent with many studies conducted in some areas of the entire country, which confirmed that most of the population and different age groups suffer from a deficiency in the level of vitamin D and calcium, despite the country's location in an environmental zone suitable for the availability of sufficient sunlight However, due to some reasons of lack of health awareness, daily lifestyle and some social and dietary habits that have further exacerbated the problem of the spread of vitamin D and calcium deficiency among the population, the study recommended conducting intensive research and studies in various regions and following up on the cases of the population, spreading health awareness through various available means and holding scientific seminars and conferences for specialists, to educate society about the dangers of vitamin D and calcium deficiency on public health Changing habits and daily life patterns, conducting routine examinations, and following a healthy diet to reduce the increasing shortage rates among different age groups of the population.

While most pregnant women in Gharyan city demonstrated adequate oral health knowledge, gaps persist in

preventive practices, particularly regarding fluoride use and dental visits. Education level and dental visits

during pregnancy were significant determinants of knowledge. These findings highlight the need for targeted

oral health education programs and increased access to dental services for pregnant women to improve oral

health outcomes for both mothers and their infants.

Abstract Classroom presentations represent an important aspect of activities that help English as a Second Language learners showcase their language skills, particularly speaking in front of others, and reveal the educational and psychological obstacles and challenges they face in performing these presentations at the best possible level. The study aims to identify the impact of language anxiety on classroom presentations by applying it to English as Foreign Language learners at Gharian University, which helps to investigate the level and type of anxiety that English as a Foreign Language students at the university suffer from during presentations. The study was conducted on various students of different semesters at University. The sample consisted of 100 students of both genders, divided into two groups (45 males and 45 females), during the academic year 2025-2026. The study relied on a questionnaire as the data collection tool, and the collected data was statistically analyzed using SPSS software. The results showed a high level of anxiety about learning English among the participating students for various reasons. A statistically significant inverse relationship was found between the level of English language anxiety among University students and the quality of their classroom presentations. Statistically significant differences were also found in language anxiety levels among students during classroom presentations, while no statistically significant differences were found between gender and anxiety levels in terms of their impact on the quality of classroom presentations. The stuconcluded with recommendations and suggestions to enhance the

Abstract Antimicrobial agents are widely used to treat bacterial infections; however, their misuse contributes to the growing problem of antibiotic resistance. This study assessed the prevalence of antibiotic resistance among bacterial isolates from patients in Gharyan City, Libya, and explored behavioral factors influencing resistance. Between January and June 2024, 66 clinical samples (urine, stool, vaginal swabs, blood, pus, and sputum) were collected from seven medical laboratories. A questionnaire survey was conducted among three groups: medical workers (n=50), non-medical individuals (n=50), and pharmacists (n=50). Laboratory identification employed selective media, Gram staining, and microscopy, while antibiotic susceptibility was tested using disc diffusion. Results revealed widespread inappropriate antibiotic use: 70% of pharmacists dispensed antibiotics without prescriptions, 40% provided incorrect dosages, and only 10% held formal pharmacy qualifications. Among medical workers, 40% used antibiotics without prescriptions and 56% failed to complete treatment courses. Infections were more common in females (77%) than males (23%), with the highest prevalence in younger age groups (<32 years, 39.4%). Staphylococcus spp (32%) and E. coli (27%) were the most frequent isolates. Overall resistance was high (75%), particularly in Pseudomonas spp (100%), E. coli (83%), and Staphylococcus spp (76%). Azithromycin (67%) and Doxycycline (49%) showed the highest resistance rates, while Meropenem (2%) and Nalidixic acid (3%) remained largely effective. These findings highlight alarming levels of antibiotic resistance in Gharyan, driven by misuse and poor prescribing practices. Strengthening pharmacist and physician training, enforcing prescription regulations, and raising public awareness are essential to mitigate resistance and preserve antibiotic efficacy. Keywords. Antibiotic Resistance, Bacterial Infection, Misuse of Antibiotics, Control and Surveillance.

Abstract

Stem cell therapy has emerged as a promising approach for craniofacial bone remodeling and dental tissue regeneration. In this study, a mixed-methods design was employed, combining laboratory analyses with clinical data from 100 participants. Results demonstrated that 80% of respondents had received mesenchymal stem cell (MSC) therapy, with 70% reporting improvements in bone density and 65% noting enhanced dental tissue regeneration. Imaging tests confirmed progress in 85% of cases, while overall satisfaction with therapy outcomes was reported by 80–85% of participants. Correlation analysis revealed strong positive relationships between stem cell treatment and improvements in bone and dental tissues, with coefficients ranging from 0.60 to 0.70 (p < 0.01). Side effects were minimal, with only 20% reporting complications such as swelling or pain. These findings underscore the therapeutic potential of stem cell-based interventions, highlighting both their efficacy and safety. The study concludes that stem cell therapy represents a viable and effective strategy for regenerative dentistry and craniofacial surgery, though larger multicenter trials are needed to standardize protocols and validate long-term outcomes.

Abstract

Periodontal disease is a multifactorial condition characterized by chronic inflammation and progressive destruction of supporting tissues, with significant implications for oral health and systemic well-being. This study investigated the correlation between inflammatory responses and regenerative outcomes in periodontal supporting tissues, emphasizing the role of epigenetic regulation. A mixed methods approach was employed, combining clinical assessments, patient-reported outcomes, and molecular analyses. Spearman correlation analysis revealed strong associations between clinical indicators of inflammation and perceived regenerative improvements, underscoring the interplay between destructive and reparative processes. Demographic and lifestyle factors, including smoking and systemic health conditions, were found to influence variability in responses. Regenerative therapies demonstrated favorable outcomes in terms of clinical attachment gain, alveolar bone support, and patient satisfaction. These findings highlight the importance of integrating epigenetic insights with clinical practice to develop personalized strategies for managing periodontal disease and enhancing tissue regeneration

ABSTRACT This study investigates the effects of self-assessment on students’ motivation and language proficiency among English Department students at the Faculty of Arts, AL-Assaba. A quantitative research design was adopted, using a questionnaire distributed to a sample of 60 students. The findings indicate that self-assessment plays a significant role in enhancing learners’ motivation, self-awareness, and perceived language proficiency. Most students reported positive attitudes toward self-assessment and felt it Improved their English skills. The study concludes that incorporating self-assessment into English language instruction can foster learner autonomy and Improve learning outcomes. It recommends Integrating self-assessment practices into curricula and providing guidance to students on effective self-assessment. Keywords: Self-assessment, Motivation, Language Proficiency, Learner Autonomy, English Department Students, Higher Education.