While most pregnant women in Gharyan city demonstrated adequate oral health knowledge, gaps persist in

preventive practices, particularly regarding fluoride use and dental visits. Education level and dental visits

during pregnancy were significant determinants of knowledge. These findings highlight the need for targeted

oral health education programs and increased access to dental services for pregnant women to improve oral

health outcomes for both mothers and their infants.

ABSTRACT This study investigates the effects of self-assessment on students’ motivation and language proficiency among English Department students at the Faculty of Arts, AL-Assaba. A quantitative research design was adopted, using a questionnaire distributed to a sample of 60 students. The findings indicate that self-assessment plays a significant role in enhancing learners’ motivation, self-awareness, and perceived language proficiency. Most students reported positive attitudes toward self-assessment and felt it Improved their English skills. The study concludes that incorporating self-assessment into English language instruction can foster learner autonomy and Improve learning outcomes. It recommends Integrating self-assessment practices into curricula and providing guidance to students on effective self-assessment. Keywords: Self-assessment, Motivation, Language Proficiency, Learner Autonomy, English Department Students, Higher Education.  

Abstract

Periodontal disease is a multifactorial condition characterized by chronic inflammation and progressive destruction of supporting tissues, with significant implications for oral health and systemic well-being. This study investigated the correlation between inflammatory responses and regenerative outcomes in periodontal supporting tissues, emphasizing the role of epigenetic regulation. A mixed methods approach was employed, combining clinical assessments, patient-reported outcomes, and molecular analyses. Spearman correlation analysis revealed strong associations between clinical indicators of inflammation and perceived regenerative improvements, underscoring the interplay between destructive and reparative processes. Demographic and lifestyle factors, including smoking and systemic health conditions, were found to influence variability in responses. Regenerative therapies demonstrated favorable outcomes in terms of clinical attachment gain, alveolar bone support, and patient satisfaction. These findings highlight the importance of integrating epigenetic insights with clinical practice to develop personalized strategies for managing periodontal disease and enhancing tissue regeneration

Abstract

Natural dentin repair is a biologically mediated process that preserves tooth vitality through odontoblast activity and intracellular signaling pathways. This study employed a mixed methods design, combining laboratory experiments with descriptive analytical surveys of dentists in Egypt, to investigate the mechanisms of odontoblast differentiation and assess clinical knowledge and practices related to regenerative dentistry. Laboratory analyses included microscopic and molecular techniques to evaluate cellular signaling during dentin repair, while questionnaires were distributed to 100 dentists across public and private sectors. Data were analyzed using quantitative statistics, qualitative assessments, and integrative interpretation of laboratory and clinical findings. Results revealed significant positive correlations between dentists’ knowledge of intracellular signaling and their clinical practices, with Spearman correlation coefficients ranging from 0.52 to 0.65 (p = 0.01). A majority of respondents (80%) reported experience with mesenchymal stem cell therapy for craniofacial bone remodeling or dental tissue regeneration, and 70% observed improvements in bone density, while 65% reported enhanced dental tissue regeneration. Imaging studies were widely used, with 85% of participants undergoing radiographic or CT evaluations to monitor progress. Despite these encouraging outcomes, only 35% of dentists were familiar with molecular techniques targeting signaling pathways, and just 40% reported applying clinical strategies to enhance odontoblast differentiation. Notably, 75% expressed interest in specialized training to improve their knowledge and practices in regenerative dentistry. The study concludes that while theoretical awareness of dentin repair mechanisms is relatively strong, clinical application remains limited. Bridging this gap through structured training and evidence-based protocols will be essential for integrating molecular insights into routine dental practice, thereby improving dentin repair outcomes and long-term tooth vitality.

Abstract

Stem cell therapy has emerged as a promising approach for craniofacial bone remodeling and dental tissue regeneration. In this study, a mixed-methods design was employed, combining laboratory analyses with clinical data from 100 participants. Results demonstrated that 80% of respondents had received mesenchymal stem cell (MSC) therapy, with 70% reporting improvements in bone density and 65% noting enhanced dental tissue regeneration. Imaging tests confirmed progress in 85% of cases, while overall satisfaction with therapy outcomes was reported by 80–85% of participants. Correlation analysis revealed strong positive relationships between stem cell treatment and improvements in bone and dental tissues, with coefficients ranging from 0.60 to 0.70 (p < 0.01). Side effects were minimal, with only 20% reporting complications such as swelling or pain. These findings underscore the therapeutic potential of stem cell-based interventions, highlighting both their efficacy and safety. The study concludes that stem cell therapy represents a viable and effective strategy for regenerative dentistry and craniofacial surgery, though larger multicenter trials are needed to standardize protocols and validate long-term outcomes.

Antimicrobial Susceptibility Testing (AST) is essential for guiding appropriate antibiotic therapy. The disk diffusion method is widely used in Libya due to its low cost, yet deviations from standardized protocols can compromise result accuracy, affecting clinical decisions and patient outcomes. This study evaluated the performance of the disk diffusion AST method in medical laboratories in Gharyan, Libya, using EUCAST guidelines (version 12.0) as a benchmark. Seventy-five AST plates representing 122 clinical specimens were randomly collected from three pathology centers between September and November 2025. Plates were analyzed for the type of culture media, number of specimens per plate, number of antibiotic disks, and readability of results. All plates were 90 mm in diameter. Nutrient agar was used for 118 specimens, and blood agar for two; none used Mueller-Hinton agar as recommended. Only 17% of plates tested a single specimen per plate; the majority tested one specimen on half or a quarter of a plate. The number of antibiotic disks per specimen often exceeded EUCAST limits (up to 20 disks per plate), and only 8% of plates yielded readable results. Widespread deviations from EUCAST protocols—including inappropriate media, specimen overloading, and excessive disks—compromise AST reliability. These errors may lead to incorrect antibiotic prescriptions, delayed recovery, and increased antimicrobial resistance. Strict adherence to standardized guidelines is urgently required to ensure accurate, clinically meaningful AST results. Moreover, raising awareness of the importance of following standard guidelines is crucial for correcting the course

Abstract

The proper management of biological waste in laboratory settings is a critical aspect of biosafety in microbiological and biomedical laboratories. Beyond protecting lab workers, biological waste management safeguards public health, protects the environment, and ensures compliance with legal and regulatory frameworks. Segregation, decontamination of liquid waste, decontamination of solid waste, and techniques for inactivating toxins are all main aspects of biological waste management that will be outlined, aligned, and summarized with the main institutional biosafety protocols for biological waste management, published by major authorities, including CDC and WHO. This research found that the information from the two institutions complements each other. Therefore, this brief review aimed to compile and unify information from both institutions to construct a single, coherent text that provides a practical summary for medical laboratory professionals seeking to enhance the implementation of international, harmonized protocols that strengthen biosafety, environmental stewardship, and regulatory compliance