Abstract

Natural dentin repair is a biologically mediated process that preserves tooth vitality through odontoblast activity and intracellular signaling pathways. This study employed a mixed methods design, combining laboratory experiments with descriptive analytical surveys of dentists in Egypt, to investigate the mechanisms of odontoblast differentiation and assess clinical knowledge and practices related to regenerative dentistry. Laboratory analyses included microscopic and molecular techniques to evaluate cellular signaling during dentin repair, while questionnaires were distributed to 100 dentists across public and private sectors. Data were analyzed using quantitative statistics, qualitative assessments, and integrative interpretation of laboratory and clinical findings. Results revealed significant positive correlations between dentists’ knowledge of intracellular signaling and their clinical practices, with Spearman correlation coefficients ranging from 0.52 to 0.65 (p = 0.01). A majority of respondents (80%) reported experience with mesenchymal stem cell therapy for craniofacial bone remodeling or dental tissue regeneration, and 70% observed improvements in bone density, while 65% reported enhanced dental tissue regeneration. Imaging studies were widely used, with 85% of participants undergoing radiographic or CT evaluations to monitor progress. Despite these encouraging outcomes, only 35% of dentists were familiar with molecular techniques targeting signaling pathways, and just 40% reported applying clinical strategies to enhance odontoblast differentiation. Notably, 75% expressed interest in specialized training to improve their knowledge and practices in regenerative dentistry. The study concludes that while theoretical awareness of dentin repair mechanisms is relatively strong, clinical application remains limited. Bridging this gap through structured training and evidence-based protocols will be essential for integrating molecular insights into routine dental practice, thereby improving dentin repair outcomes and long-term tooth vitality.

Abstract

Stem cell therapy has emerged as a promising approach for craniofacial bone remodeling and dental tissue regeneration. In this study, a mixed-methods design was employed, combining laboratory analyses with clinical data from 100 participants. Results demonstrated that 80% of respondents had received mesenchymal stem cell (MSC) therapy, with 70% reporting improvements in bone density and 65% noting enhanced dental tissue regeneration. Imaging tests confirmed progress in 85% of cases, while overall satisfaction with therapy outcomes was reported by 80–85% of participants. Correlation analysis revealed strong positive relationships between stem cell treatment and improvements in bone and dental tissues, with coefficients ranging from 0.60 to 0.70 (p < 0.01). Side effects were minimal, with only 20% reporting complications such as swelling or pain. These findings underscore the therapeutic potential of stem cell-based interventions, highlighting both their efficacy and safety. The study concludes that stem cell therapy represents a viable and effective strategy for regenerative dentistry and craniofacial surgery, though larger multicenter trials are needed to standardize protocols and validate long-term outcomes.

While most pregnant women in Gharyan city demonstrated adequate oral health knowledge, gaps persist in

preventive practices, particularly regarding fluoride use and dental visits. Education level and dental visits

during pregnancy were significant determinants of knowledge. These findings highlight the need for targeted

oral health education programs and increased access to dental services for pregnant women to improve oral

health outcomes for both mothers and their infants.

Abstract Antimicrobial agents are widely used to treat bacterial infections; however, their misuse contributes to the growing problem of antibiotic resistance. This study assessed the prevalence of antibiotic resistance among bacterial isolates from patients in Gharyan City, Libya, and explored behavioral factors influencing resistance. Between January and June 2024, 66 clinical samples (urine, stool, vaginal swabs, blood, pus, and sputum) were collected from seven medical laboratories. A questionnaire survey was conducted among three groups: medical workers (n=50), non-medical individuals (n=50), and pharmacists (n=50). Laboratory identification employed selective media, Gram staining, and microscopy, while antibiotic susceptibility was tested using disc diffusion. Results revealed widespread inappropriate antibiotic use: 70% of pharmacists dispensed antibiotics without prescriptions, 40% provided incorrect dosages, and only 10% held formal pharmacy qualifications. Among medical workers, 40% used antibiotics without prescriptions and 56% failed to complete treatment courses. Infections were more common in females (77%) than males (23%), with the highest prevalence in younger age groups (<32 years, 39.4%). Staphylococcus spp (32%) and E. coli (27%) were the most frequent isolates. Overall resistance was high (75%), particularly in Pseudomonas spp (100%), E. coli (83%), and Staphylococcus spp (76%). Azithromycin (67%) and Doxycycline (49%) showed the highest resistance rates, while Meropenem (2%) and Nalidixic acid (3%) remained largely effective. These findings highlight alarming levels of antibiotic resistance in Gharyan, driven by misuse and poor prescribing practices. Strengthening pharmacist and physician training, enforcing prescription regulations, and raising public awareness are essential to mitigate resistance and preserve antibiotic efficacy. Keywords. Antibiotic Resistance, Bacterial Infection, Misuse of Antibiotics, Control and Surveillance.

Abstract

Periodontal disease is a multifactorial condition characterized by chronic inflammation and progressive destruction of supporting tissues, with significant implications for oral health and systemic well-being. This study investigated the correlation between inflammatory responses and regenerative outcomes in periodontal supporting tissues, emphasizing the role of epigenetic regulation. A mixed methods approach was employed, combining clinical assessments, patient-reported outcomes, and molecular analyses. Spearman correlation analysis revealed strong associations between clinical indicators of inflammation and perceived regenerative improvements, underscoring the interplay between destructive and reparative processes. Demographic and lifestyle factors, including smoking and systemic health conditions, were found to influence variability in responses. Regenerative therapies demonstrated favorable outcomes in terms of clinical attachment gain, alveolar bone support, and patient satisfaction. These findings highlight the importance of integrating epigenetic insights with clinical practice to develop personalized strategies for managing periodontal disease and enhancing tissue regeneration

Abstract: 

This study presents a critical discourse analysis (CDA) of the construction of political identity 

during periods of revolutionary crisis. It examines the dichotomous representation of Muammar 

Gaddafi in February 2011 through two primary data streams: his first major public address 

following the uprising (February 22, 2011) and the contemporaneous visual and textual 

coverage in three newspapers—The Sun (UK), The Daily News (a prominent US tabloid-style 

outlet), and Alshourouq (Egypt). Employing Fairclough’s (1992) three-dimensional model of 

CDA, Halliday’s (1978) Systemic Functional Linguistics (SFL), and Kress and van Leeuwen’s 

(2006) grammar of visual design, the analysis reveals a profound disjuncture between Gaddafi’s 

linguistic self-fashioning and his mediated portrayal. Findings indicate that Gaddafi’s speech 

strategically deployed lexical choices, transitivity, and modality to construct identities of a 

rightful leader, paternal protector, and historical embodiment of Libya. Conversely, the selected 

newspapers, despite ideological nuances, converged on a representational framework depicting 

Gaddafi as weak, cowardly, and delegitimized, primarily through specific visual framing, 

camera angles, and captions. This study argues that the conflict between these representations 

is not merely descriptive but deeply ideological, reflecting the struggle for discursive hegemony 

during the Arab Spring, where control over narrative became a key battleground alongside 

military confrontation.

Background and objective :Helicobacter pylori [H.pylori] infection is one of the most common chronic bacterial infections worldwide especially in the developing countries. This bacterium is the cause of many diseases such as MALT lymphoma, gastritis, peptic ulcers, and stomach cancer. According to recent reports, H.pylori infection may increase the risk of colorectal cancer. The current study aimed at investigating the association of H. pylori infection and the risk of colorectal cancer and adenomatous polyps. Methods: The current study was conducted on 52 patients with colon cancer as the case group and 200 subjects without pathological finding (i e, polyps, neoplasms, or inflammatory diseases) as the control group. Blood samples were collected from the patients in order to assess the presence of anti-H. pylori infection antibodies by the serum titer levels of anti-H. pylori IgG antibodies were measured using enzyme-linked immunosorbent assay (ELISA) with commercial kit by (Dia.Pro Diagnostic Bioprobes-Italy). Results: This study demonstrates distinct associations between H.pylori infection markers and CRC risk. While IgG seropositivity showed a significant 2.16-fold increased CRC risk (p = 0.019). Conclusion: This research finding IgG seropositivity may serve as a broader risk marker.