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Clostridioides difficile is widely recognised as one of the most common causes of healthcare-associated C. difficile infections due to the ability of spores to survive for prolonged periods in the hospital environment. This study aimed to evaluate the efficacy of UV-C 254 nm light in the inactivation of the spores of different C. difficile ribotypes on brain heart infusion (BHI) agar plates or in phosphate-buffered saline (PBS) with varying spore densities. Furthermore, the effectiveness of a sporicidal surface disinfectant against C. difficile spores was determined on different surfaces. Spore suspensions of different C. difficile strains in the range of 10⁵–10⁷ colony-forming units (CFUs) mL⁻¹ were inoculated on BHI agar plates or in PBS and exposed to UV-C light for up to 30 min. Additionally, a spore suspension of 10³–10⁵ CFUs was spread over a 1 cm² test area on different surfaces, and sporicidal surface wipes were used according to the manufacturer’s instructions. The findings demonstrated that spores of C. difficile ribotypes exhibited a complete reduction in log₁₀ CFU on BHI agar plates and PBS following 20 min of exposure to a UV-C dose of 2208 mJ cm⁻². The surface wipes with sporicidal properties demonstrated efficacy in reducing the number of C. difficile spores on the Formica, stainless steel, and plastic surfaces by 2.03–3.53 log₁₀. The present study demonstrates that moist surfaces or liquids can enhance the efficacy of UV-C treatment in reducing C. difficile spores. This approach may be applicable to the surfaces of healthcare facilities and to water disinfection systems.

Abstract

For the first time in Libyan flora, a new record for Boerhavia repens L is recorded. This widespread wild weed was collected from the Ariggiba region (110 km west-south Sabha city). A full description, habitat information, distribution map, and distribution data are provided to facilitate further identification and future detection. A brief discussion about the most important threats this species poses is presented.

:Abstract

This project includes and in deification the complexes of amino acid L-serine (serH) L-cysteine (cysH) , and L-threonin (thrH₂) with some ions of trace elements such as K₂ptCl₆ ,

Cu(NO₃)₂.3H₂O , ZnCl₂ and HgCl₂ were studied by Infrared-spectra ‘NMR from this identification studies can give the structure formed for the complexes with were prepare with amino acids :

Na[pt(ser)Cl₄] .0.5C₂H₅OH   ,[Hg(ser)₂] .9H₂O , [Cu(ser)₂] , Na[pt(cys)₂Cl₃].0.5C₂H₅OH.3H₂O and

Keywords: metal complexes – Amino acids – Characterization .

الملخص:                                                                                                                                                              تم في هذا البحث تحضير وتشخيص بعض المعقدات من تفاعل بعض من ايونات العناصر هي : Z(II) , Cu(II) , Pt(IV) , Hg(II)    مع الأحماض الأمينية مثل السستايين و السيرين و الثرونين و التي شخصت بعدة طرق منها طيف الأشعة تحت الحمراء والمرئية وفوق البنفسجية بالأضافة الى استعمال الطرق الفيزيائية الأتية : درجة الأنصهار والذائيبية والتوصيل الكهربائي لمحاليلها .

الكلمات المفتاحية : الأحماض الأمينية – المعقدات – التشخيص