Antimicrobial Susceptibility Testing (AST) is essential for guiding appropriate antibiotic therapy. The disk diffusion method is widely used in Libya due to its low cost, yet deviations from standardized protocols can compromise result accuracy, affecting clinical decisions and patient outcomes. This study evaluated the performance of the disk diffusion AST method in medical laboratories in Gharyan, Libya, using EUCAST guidelines (version 12.0) as a benchmark. Seventy-five AST plates representing 122 clinical specimens were randomly collected from three pathology centers between September and November 2025. Plates were analyzed for the type of culture media, number of specimens per plate, number of antibiotic disks, and readability of results. All plates were 90 mm in diameter. Nutrient agar was used for 118 specimens, and blood agar for two; none used Mueller-Hinton agar as recommended. Only 17% of plates tested a single specimen per plate; the majority tested one specimen on half or a quarter of a plate. The number of antibiotic disks per specimen often exceeded EUCAST limits (up to 20 disks per plate), and only 8% of plates yielded readable results. Widespread deviations from EUCAST protocols—including inappropriate media, specimen overloading, and excessive disks—compromise AST reliability. These errors may lead to incorrect antibiotic prescriptions, delayed recovery, and increased antimicrobial resistance. Strict adherence to standardized guidelines is urgently required to ensure accurate, clinically meaningful AST results. Moreover, raising awareness of the importance of following standard guidelines is crucial for correcting the course

Despite the critical demand for advanced English proficiency in Libyan 

higher education, primary public schools, particularly in rural areas, have 

been neglected. This qualitative study investigates the imperative for 

implementing improved methodologies in Teaching English as a Foreign 

Language (EFL) in Libyan primary public schools, using the post-conflict 

rural municipality of Al Gwaleesh as a case study. Data were collected by 

questionnaires and classroom observations from ten teachers and ten third

year pupils. The findings reveal a systemic reliance on traditional Grammar

Translation Methods, a stark deficiency of resources (including basic 

teaching aids and ICT), and minimal student engagement. Teachers 

demonstrated limited application of Communicative Language Teaching 

(CLT) principles, focusing predominantly on rote memorization and written 

exercises. Consequently, pupils reported low motivation and a failure to 

grasp the language's practical utility. The study concludes that a 

fundamental overhaul of EFL pedagogy in Libya is urgently required, 

recommending the integration of audio-visual aids, activity-based learning, 

and 

strategic 

incorporation 

of 

Information and Communication 

Technologies (ICTs) to foster intrinsic motivation and build a sustainable 

foundation for English proficiency.

Background and objective :Helicobacter pylori [H.pylori] infection is one of the most common chronic bacterial infections worldwide especially in the developing countries. This bacterium is the cause of many diseases such as MALT lymphoma, gastritis, peptic ulcers, and stomach cancer. According to recent reports, H.pylori infection may increase the risk of colorectal cancer. The current study aimed at investigating the association of H. pylori infection and the risk of colorectal cancer and adenomatous polyps. Methods: The current study was conducted on 52 patients with colon cancer as the case group and 200 subjects without pathological finding (i e, polyps, neoplasms, or inflammatory diseases) as the control group. Blood samples were collected from the patients in order to assess the presence of anti-H. pylori infection antibodies by the serum titer levels of anti-H. pylori IgG antibodies were measured using enzyme-linked immunosorbent assay (ELISA) with commercial kit by (Dia.Pro Diagnostic Bioprobes-Italy). Results: This study demonstrates distinct associations between H.pylori infection markers and CRC risk. While IgG seropositivity showed a significant 2.16-fold increased CRC risk (p = 0.019). Conclusion: This research finding IgG seropositivity may serve as a broader risk marker.

Abstract: 

This study presents a critical discourse analysis (CDA) of the construction of political identity 

during periods of revolutionary crisis. It examines the dichotomous representation of Muammar 

Gaddafi in February 2011 through two primary data streams: his first major public address 

following the uprising (February 22, 2011) and the contemporaneous visual and textual 

coverage in three newspapers—The Sun (UK), The Daily News (a prominent US tabloid-style 

outlet), and Alshourouq (Egypt). Employing Fairclough’s (1992) three-dimensional model of 

CDA, Halliday’s (1978) Systemic Functional Linguistics (SFL), and Kress and van Leeuwen’s 

(2006) grammar of visual design, the analysis reveals a profound disjuncture between Gaddafi’s 

linguistic self-fashioning and his mediated portrayal. Findings indicate that Gaddafi’s speech 

strategically deployed lexical choices, transitivity, and modality to construct identities of a 

rightful leader, paternal protector, and historical embodiment of Libya. Conversely, the selected 

newspapers, despite ideological nuances, converged on a representational framework depicting 

Gaddafi as weak, cowardly, and delegitimized, primarily through specific visual framing, 

camera angles, and captions. This study argues that the conflict between these representations 

is not merely descriptive but deeply ideological, reflecting the struggle for discursive hegemony 

during the Arab Spring, where control over narrative became a key battleground alongside 

military confrontation.

تقدم هذه الورقة بحثاً حول إطار عمل يعتمد على التعلم العميق للتصنيف متعدد الملصقات لأمراض الصدر باستخدام صور الأشعة السينية وبنية EfficientNet. يعالج هذا الإطار تحدي تشخيص الحالات المتعددة التي تتشابه في المظهر وغالباً ما تحدث معاً. يستخدم إطار العمل تقنية التعلم بنقل الخبرة (Transfer Learning)، مع إجراء ضبط دقيق لعدة متغيرات من نموذج EfficientNet (من B0 إلى B4) باستخدام مجموعة بيانات NIH ChestX-ray14. كما يوظف البحث تقنيات Focal Loss و Stochastic Weight Averaging لمعالجة اختلال توازن البيانات وتحسين قدرة النموذج على التعميم. يعتمد هذا النهج على آليات الانتباه (Attention Mechanisms) وتقنية Grad-CAM لجعل النتائج أكثر قابلية للتفسير، مما يثبت أن التوقعات تستند إلى ميزات سريرية هامة. حقق النموذج درجات عالية في مقياس ROC-AUC للحالات الأكثر وضوحاً مثل الانصباب (0.8545)، وتضخم القلب (0.8003)، والاسترواح الصدري (0.7820). ومع ذلك، واجه النموذج صعوبة أكبر في تصنيف الحالات الأكثر دقة مثل الارتشاح، والعقيدات، والالتهاب الرئوي. يؤدي إطار العمل بشكل يضاهي أو يتفوق على العديد من نماذج الشبكات العصبية التلافيفية (CNN) القائمة من حيث الدقة والكفاءة. يوفر هذا البحث سير عمل قوياً وقابلاً للتكرار لتطبيقات الأشعة، مع إمكانية استخدامه في مهام تشخيصية أخرى. كما تشير الدراسة إلى بعض المعوقات، مثل اختلال توازن الفئات والحاجة إلى دعم أفضل لاتخاذ القرارات السريرية، وتقترح تحسينات مستقبلية مثل تقنيات أفضل لتعزيز البيانات ونمذجة عدم اليقين.

This study focused on documenting flowering plant species in Wadi Al-Yaquib in Gharyan City . The plants were collected between March and May 2024 , dtied, and transferred to the herbarium for classification, During field visits,42 plant species were collected and classified, belonging to 40 genera distributed across 21 families. Of these, and 34 genera were classified under 34 dicotyledonous species, represnting 85 % of the total, and distributed across 18 families, Also 6 genera were classified under speceis of monocotyledonous plants, These species reprsent a percentage of 15% distributed over a number of three plant families, The analysis of flora in relation to the total number of species and genera belonging to each family revealed that the family. Astraceae was the largest family in class dicotyledonus opsida As a comparsion between the current study and the previous studies, this study drew attention to a plant species potentially endangered due to environmental pressures and overgrazing.

Abstract

The proper management of biological waste in laboratory settings is a critical aspect of biosafety in microbiological and biomedical laboratories. Beyond protecting lab workers, biological waste management safeguards public health, protects the environment, and ensures compliance with legal and regulatory frameworks. Segregation, decontamination of liquid waste, decontamination of solid waste, and techniques for inactivating toxins are all main aspects of biological waste management that will be outlined, aligned, and summarized with the main institutional biosafety protocols for biological waste management, published by major authorities, including CDC and WHO. This research found that the information from the two institutions complements each other. Therefore, this brief review aimed to compile and unify information from both institutions to construct a single, coherent text that provides a practical summary for medical laboratory professionals seeking to enhance the implementation of international, harmonized protocols that strengthen biosafety, environmental stewardship, and regulatory compliance